IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity
- Authors
- Hue, J; Kim, A; Song, H; Choi, I; Park, H; Kim, T; Lee, WJ; Kang, H; Cho, D
- Issue Date
- Jan-2005
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- IL-18; SCF melanoma; ROI; p38 MAPK
- Citation
- IMMUNOLOGY LETTERS, v.96, no.2, pp 211 - 217
- Pages
- 7
- Journal Title
- IMMUNOLOGY LETTERS
- Volume
- 96
- Number
- 2
- Start Page
- 211
- End Page
- 217
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15547
- DOI
- 10.1016/j.imlet.2004.08.008
- ISSN
- 0165-2478
1879-0542
- Abstract
- It has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-L-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells. (C) 2004 Elsevier B.V. All rights reserved.
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