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Transcriptional regulation of glutathione synthetase in the fission yeast Schizosaccharomyces pombe

Authors
Kim, SJKim, HGKim, BCPark, EHLim, CJ
Issue Date
Oct-2004
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Keywords
carbon source; fission yeast; glutathione synthetase; menadione; nitric oxide; Schizosaccharomyces pombe; transcriptional regulation
Citation
MOLECULES AND CELLS, v.18, no.2, pp 242 - 248
Pages
7
Journal Title
MOLECULES AND CELLS
Volume
18
Number
2
Start Page
242
End Page
248
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15817
ISSN
1016-8478
0219-1032
Abstract
Glutathione (GSH), an important antioxidant involved in the stress response, is synthesized in two sequential reactions involving glutamylcysteine synthetase (GCS), followed by glutathione synthetase (GS). Expression of the unique GS gene in the fission yeast Schizosaccharomyces pombe was previously found to be regulated by nitric oxide and by L-buthionine-(S,R)sulfoximine (BSO), a specific inhibitor of GCS. In this work, expression of S. pombe GS gene is shown to be induced by menadione (MD), which generates superoxide. The responsible DNA sequence between -365 and -234 bp from the translation start site, was convinced using five GS-lacZ fusion plasmids. Expression of GS gene is also induced by low glucose, fructose and disaccharides, apparently dependent on Pap1 protein; GS mRNA increases in low concentrations of glucose in wild type S. pombe but not in Pap1-negative cells. Although nonfermentable carbon sources such as acetate and ethanol stimulate expression of GS gene, they also arrest the growth of the yeast cells. These results indicate that the biosynthesis of glutathione is regulated by superoxide radicals and carbon source limitation.
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