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Effects of major dietary antioxidants on inflammatory markers of RAW 264.7 macrophages

Authors
Jung, WJSung, MK
Issue Date
2004
Publisher
WILEY
Keywords
antioxidants; inflammation; macrophages; inflammatory cytokines; COX-2; iNOS
Citation
BIOFACTORS, v.21, no.1-4, pp.113 - 117
Journal Title
BIOFACTORS
Volume
21
Number
1-4
Start Page
113
End Page
117
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16131
DOI
10.1002/biof.552210122
ISSN
0951-6433
Abstract
Oxidative stresses are involoved in the process of chronic inflammatory diseases. The objective of this study was to evaluate and compare the anti-inflammatory activity of major dietary antioxidants. Murine RAW264.7 macrophages (4 X 10(6) cells) were incubated in DMEM containing 10% FBS supplemented with 5 to 20 muM of alpha-tocopherol, beta-carotene or quercetin for 24 hrs, and then treated with LPS for 16hrs. The medium was collected to measure TNF-alpha, IL-6, PGE(2) and nitrite. Expressions of cyclooxygenase-2(COX-2) and inducible nitric oxide synthase(iNOS) were determined using the harvested cells. Results indicate that TNF-alpha and IL-6 accumulations were significantly reduced by 5 to 20 muM quercetin treatment, and 20 muM of alpha-tocopherol treatment. Nitrite release was significantly reduced by 5 muM quercetin treatment. However, PGE(2) accumulation was not affected by any of the antioxidants used. Expressions of COX-2 and iNOS were effectively reduced by 5 muM quercetin treatment. These findings indicate that dietary antioxidants possess significant anti-inflammatory activities, and quercetin is the most potent antioxidant.
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