Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Molecular cloning and analysis of the aroD gene encoding 3-dehydroquinate dehydratase from Corynebacterium glutamicum

Full metadata record
DC FieldValueLanguage
dc.contributor.authorPark, KY-
dc.contributor.authorLee, JS-
dc.contributor.authorChoi, SY-
dc.contributor.authorCho, DH-
dc.contributor.authorCheon, CI-
dc.contributor.authorSong, E-
dc.contributor.authorMin, KH-
dc.contributor.authorChung, H-
dc.contributor.authorLee, HS-
dc.contributor.authorLee, MS-
dc.date.available2021-02-22T16:17:01Z-
dc.date.issued2003-12-
dc.identifier.issn0254-5934-
dc.identifier.urihttps://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16147-
dc.description.abstractThe aroD gene was cloned from Corynebacterium glutamicum by complementation of Escherichia coli aromatic amimo acid auxotroph. The E. coli aroD gene codes for 3-dehydroquinte dehydratase (dehydroquinase), an enzyme involved in the aromatic amino acid biosynthesis pathway. The cloned insert DNA fragments in the recombinant plasmids, named pROD5, pROD8, and pROD14, were physically mapped with several restriction endonucleases. We determined the DNA nucleotide sequences of the fragment and reported here the sequences of the aroD gene. The complete nucleotide sequences of the aroD gene showed an open reading frame encoding a protein of 145 amino acids with a deduced molecular weight of about 16 kDa. Comparison of this deduced amino acid sequences of C. glutamicum aroD gene with those of the enzyme from Pseudomonas aeruginosa, Mycobacterium tuberculosis and Actinobacillus pleuropneumonia revealed 51%, 53% and 54% similarity, respectively.-
dc.format.extent9-
dc.language영어-
dc.language.isoENG-
dc.publisherGENETICS SOC KOREA-
dc.titleMolecular cloning and analysis of the aroD gene encoding 3-dehydroquinate dehydratase from Corynebacterium glutamicum-
dc.typeArticle-
dc.publisher.location대한민국-
dc.identifier.scopusid2-s2.0-13544252748-
dc.identifier.wosid000187754100011-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF GENETICS, v.25, no.4, pp 369 - 377-
dc.citation.titleKOREAN JOURNAL OF GENETICS-
dc.citation.volume25-
dc.citation.number4-
dc.citation.startPage369-
dc.citation.endPage377-
dc.type.docTypeArticle-
dc.identifier.kciidART000863174-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskciCandi-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalResearchAreaGenetics & Heredity-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryGenetics & Heredity-
dc.subject.keywordPlusASPERGILLUS-NIDULANS-
dc.subject.keywordPlusMYCOBACTERIUM-TUBERCULOSIS-
dc.subject.keywordPlusSALMONELLA-TYPHI-
dc.subject.keywordPlusACTIVE-SITE-
dc.subject.keywordPlusDEHYDROQUINASE-
dc.subject.keywordPlusSEQUENCE-
dc.subject.keywordPlusENZYME-
dc.subject.keywordPlusPURIFICATION-
dc.subject.keywordPlusPATHWAY-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordAuthorCorynebacterium glutamicum-
dc.subject.keywordAuthoraroD gene-
dc.subject.keywordAuthor3-dehydroquinate dehydratase-
dc.subject.keywordAuthorheterolohous complementation-
dc.subject.keywordAuthoraromatic amino acid-
dc.identifier.urlhttps://kiss.kstudy.com/Detail/Ar?key=2218819-
Files in This Item
Go to Link
Appears in
Collections
대학 > 기초교양대학 > 기초교양학부 > 1. Journal Articles
이과대학 > 생명시스템학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Cheon, Choong Ill photo

Cheon, Choong Ill
이과대학 (생명시스템학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE