Isolation and analysis of the argG gene encoding argininosuccinate synthetase from Corynebacterium glutamicum
- Ko, SY; Yim, SH; Lee, HS; Lee, MS
- Issue Date
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Corynebacterium glutamicum; argG; argininosuccinate synthetase; heterologous complementation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.13, no.6, pp.949 - 954
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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- The argG gene of Corynebacterium glutamicum encoding argininosuccinate synthetase (EC6345) was cloned and sequenced. The gene was cloned by heterologous complementation of an Escherichia coli arginine auxotrophic mutant (argG(-)). The cloned DNA fragment also. complements E. coli argD, argF, and argH mutants, suggesting a clustered organization of the genes in the chromosome. The coding region-of the argG gene is 1,206 nucleotides long with a deduced molecular weight of about 44 kDa, comparable with the predicted size of the expressed protein on. the SDS-PAGE. Computer analysis revealed that the amino acid sequence of the argG gene product had a high similarity to that of Mycobacterium tuberculosis and Streptomyces clavuligerus. Two conserved sequence motifs within the ArgG appear to be ATP-binding sites which correspond to 2 of the 3 conserved regions found in sequences of all known argininosuccinate synthetases.
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