Isolation and analysis of the argG gene encoding argininosuccinate synthetase from Corynebacterium glutamicum
DC Field | Value | Language |
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dc.contributor.author | Ko, SY | - |
dc.contributor.author | Yim, SH | - |
dc.contributor.author | Lee, HS | - |
dc.contributor.author | Lee, MS | - |
dc.date.available | 2021-02-22T16:17:03Z | - |
dc.date.issued | 2003-12 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16148 | - |
dc.description.abstract | The argG gene of Corynebacterium glutamicum encoding argininosuccinate synthetase (EC6345) was cloned and sequenced. The gene was cloned by heterologous complementation of an Escherichia coli arginine auxotrophic mutant (argG(-)). The cloned DNA fragment also. complements E. coli argD, argF, and argH mutants, suggesting a clustered organization of the genes in the chromosome. The coding region-of the argG gene is 1,206 nucleotides long with a deduced molecular weight of about 44 kDa, comparable with the predicted size of the expressed protein on. the SDS-PAGE. Computer analysis revealed that the amino acid sequence of the argG gene product had a high similarity to that of Mycobacterium tuberculosis and Streptomyces clavuligerus. Two conserved sequence motifs within the ArgG appear to be ATP-binding sites which correspond to 2 of the 3 conserved regions found in sequences of all known argininosuccinate synthetases. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Isolation and analysis of the argG gene encoding argininosuccinate synthetase from Corynebacterium glutamicum | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.scopusid | 2-s2.0-0347358998 | - |
dc.identifier.wosid | 000187661400017 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.13, no.6, pp 949 - 954 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 13 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 949 | - |
dc.citation.endPage | 954 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART000922573 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | ARGININE-BIOSYNTHESIS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | MOLECULAR CHARACTERIZATION | - |
dc.subject.keywordPlus | STREPTOMYCES-CLAVULIGERUS | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | PARTIAL-PURIFICATION | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | ACETYL CYCLE | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | argG | - |
dc.subject.keywordAuthor | argininosuccinate synthetase | - |
dc.subject.keywordAuthor | heterologous complementation | - |
dc.identifier.url | http://kiss.kstudy.com/thesis/thesis-view.asp?key=2108426 | - |
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