Purification and characterization of 2,3-dihydroxybiphenyl 1,2-dioxygenase from Comamonas sp SMN4
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, N | - |
dc.contributor.author | Lee, JM | - |
dc.contributor.author | Min, KH | - |
dc.contributor.author | Kwon, DY | - |
dc.date.available | 2021-02-22T16:17:23Z | - |
dc.date.issued | 2003-08 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16176 | - |
dc.description.abstract | 2,3-Dihydroxybiphenyl 1,2-dioxygenase (23DBDO), an enzyme of the biphenyl biodegradation pathway encoded by the bphC gene of Comamonas sp. SMN4, was expressed and purified using column chromatographies. SDS-PAGE of purified 23DBDO showed a single band with a molecular mass of 32 kDa, which was consistent with the data from the gel filtration chromatography (GFC). The purified enzyme exhibited a maximum 23DBDO activity at pH 9.0 and was stable at pH 8.0. The enzyme showed maximum activity at 40degreesC and maintained activity at 30degreesC for 24 h. Kinetic parameters represented by Michaelis-Menten constants such as K-m and V-max values for various substrates were determined by Lineweaver-Burk plots: The purified enzyme 23DBDO from Comamonas sp. SMN4 had the highest catalytic activity for 2,3-dihydroxybiphenyl and 3-methylcatechol, and had very poor activity with catechol and 4-methylcatechol. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Purification and characterization of 2,3-dihydroxybiphenyl 1,2-dioxygenase from Comamonas sp SMN4 | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.scopusid | 2-s2.0-0041377762 | - |
dc.identifier.wosid | 000185034300002 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.13, no.4, pp 487 - 494 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 13 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 487 | - |
dc.citation.endPage | 494 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART000884410 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | SP STRAIN KKS102 | - |
dc.subject.keywordPlus | PSEUDOMONAS-PSEUDOALCALIGENES | - |
dc.subject.keywordPlus | EXTRADIOL DIOXYGENASE | - |
dc.subject.keywordPlus | GENE BPHC | - |
dc.subject.keywordPlus | BIPHENYL | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | BIODEGRADATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordAuthor | dihydroxybiphenyl dioxygenase | - |
dc.subject.keywordAuthor | bphC | - |
dc.subject.keywordAuthor | Comamonas | - |
dc.subject.keywordAuthor | biphenyl degradation | - |
dc.subject.keywordAuthor | binding affinity | - |
dc.subject.keywordAuthor | substrate specificity | - |
dc.identifier.url | https://kiss.kstudy.com/Detail/Ar?key=2083760 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.