Induction of interleukin-12 production in mouse macrophages by berberine, a benzodioxoloquinolizine alkaloid, deviates CD4(+) T cells from a Th2 to a Th1 response
- Authors
- Kim, TS; Kang, BY; Cho, DH; Kim, SH
- Issue Date
- Jul-2003
- Publisher
- BLACKWELL PUBLISHING LTD
- Citation
- IMMUNOLOGY, v.109, no.3, pp 407 - 414
- Pages
- 8
- Journal Title
- IMMUNOLOGY
- Volume
- 109
- Number
- 3
- Start Page
- 407
- End Page
- 414
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16179
- DOI
- 10.1046/j.1365-2567.2003.01673.x
- ISSN
- 0019-2805
1365-2567
- Abstract
- In this study we investigated whether berberine-mediated induction of interleukin-12 (IL-12) production in antigen-presenting cells could regulate a cytokine profile of antigen-primed CD4(+) T helper (Th) cells. Pretreatment with berberine induced IL-12 production in both macrophages and dendritic cells, and significantly increased the levels of IL-12 production in lipopylysaccharide-stimulated macrophages and in CD40 ligand-stimulated dendritic cells. Importantly, berberine pretreatment of macrophages increased their ability to induce interferon-gamma (IFN-gamma) and reduced their ability to induce IL-4 in antigen-primed CD4(+) T cells. Berberine did not influence the macrophage cell surface expression of the class II major histocompatibility complex molecule, the co-stimulatory molecules CD80 and CD86, and intracellular adhesion molecule-1. Addition of neutralizing anti-IL-12p40 monoclonal antibody to cultures of berberine-pretreated macrophages and CD4(+) T cells restored IL-4 production in antigen-primed CD4(+) T cells. The In vivo administration of berberine resulted in the enhanced induction of IL-12 production by macrophages when stimulated in vitro with lipopolysaccharide or heat-killed Listeria monocytogenes, leading to the inhibition of the Th type 2 cytokine profile (decreased IL-4 and increased IFN-gamma production) in antigen-primed CD4(+) T cells. These findings may point to a possible therapeutic use of berberine or medicinal plants containing berberine in the Th type 2 cell-mediated immune diseases such as allergic diseases.
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