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Cloning and sequence analysis of two catechol-degrading gene clusters from a phenol-utilizing bacterium Pseudomonas putida SM25

Authors
Jung, YHKa, JOCheon, CILee, MSSong, ESChoi, SYCho, DChoi, SHHa, KSPark, YMChoi, JSMin, KH
Issue Date
Jun-2003
Publisher
MICROBIOLOGY SOC KOREA
Keywords
catechol degradation; catB and catC genes; cloning and sequence analysis
Citation
JOURNAL OF MICROBIOLOGY, v.41, no.2, pp.102 - 108
Journal Title
JOURNAL OF MICROBIOLOGY
Volume
41
Number
2
Start Page
102
End Page
108
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16183
ISSN
1225-8873
Abstract
A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the vector pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed active-site region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P putida SM25 were tightly linked, they could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P putida RB1.
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대학 > 기초교양대학 > 기초교양학부 > 1. Journal Articles
이과대학 > 생명시스템학부 > 1. Journal Articles

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Cheon, Choong Ill
이과대학 (생명시스템학부)
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