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Regulation of the gene encoding glutathione synthetase from the fission yeast

Authors
Kim, SJShin, YHKim, KPark, EHSa, JHLim, CJ
Issue Date
31-May-2003
Publisher
SPRINGER-VERLAG SINGAPORE PTE LTD
Keywords
fission yeast; glutathione synthetase; nitrosative stress; regulation; Schizosaccharomyces pombe; transcription
Citation
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.36, no.3, pp.326 - 331
Journal Title
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume
36
Number
3
Start Page
326
End Page
331
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16187
ISSN
1225-8687
Abstract
The fission yeast cells that contained the cloned glutathione synthetase (GS) gene showed 1.4-fold higher glutathione (GSH) content and 1.9-fold higher GS activity than the cells without the cloned GS gene. Interestingly, gamma-glutamylcysteine synthetase activity increased 2.1-fold in the S. pombe cells that contained the cloned GS gene. The S. pombe cells that harbored the multicopy-number plasmid pRGS49 (containing the cloned GS gene) showed a higher level of survival on solid media with cadmium chloride (1 mM) or mercuric chloride (10 muM) than the cells that harbored the YEp357R vector. The 506 bp upstream sequence from the translational initiation point and N-terminal 8 amino acid-coding region were fused into the promoterless beta-galactosidase gene of the shuttle vector YEp367R to generate the fusion plasmid pUGS39. Synthesis of beta-galactosidase from the fusion plasmid pUGS39 was significantly enhanced by cadmium chloride and NO-generating S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SN). It was also induced by L-buthionine-(S,R)-sulfoximine, a specific inhibitor of gamma-glutamylcysteine synthetase (GCS). We also found that the expression of the S. pombe GS gene is regulated by the Atf1-Spc1-Wis1 signal pathway.
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