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Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (bphC) from Comamonas sp. SMN4 for phylogenetic and structural analysis
- Issue Date
- Apr-2003
- Publisher
- SPRINGER-VERLAG BERLIN
- Keywords
- dihydroxybiphenyl dioxygenase; bphC; extradiol dioxygenase; Comamonas; biphenyl degradation
- Citation
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.30, no.4, pp 245 - 250
- Pages
- 6
- Journal Title
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
- Volume
- 30
- Number
- 4
- Start Page
- 245
- End Page
- 250
- ISSN
- 1367-5435
1476-5535
- Abstract
- A genomic library of biphenyl-degrading Comamonas sp. SMN4 for isolating fragments containing the 2,3-dihydroxybiphenyl 1,2-dioxygenase (23DBDO) gene was constructed. The smallest subclone (pNPX9) encoding 23DBDO activity was sequenced and analyzed. The C-terminal domain of 23DBDO from Comamonas sp. SMN4 had five catalytically essential residues and was more highly conserved than the N-terminal domain. Phylogenetic and structural relationships of 23DBDO from Comamonas sp. SMN4 were analyzed.
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