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Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (bphC) from Comamonas sp SMN4 for phylogenetic and structural analysis

Authors
Lee, NRKwon, DYMin, KH
Issue Date
Apr-2003
Publisher
SPRINGER-VERLAG BERLIN
Keywords
dihydroxybiphenyl dioxygenase; bphC; extradiol dioxygenase; Comamonas; biphenyl degradation
Citation
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.30, no.4, pp.245 - 250
Journal Title
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume
30
Number
4
Start Page
245
End Page
250
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16203
DOI
10.1007/s10295-003-0039-z
ISSN
1367-5435
Abstract
A genomic library of biphenyl-degrading Comamonas sp. SMN4 for isolating fragments containing the 2,3-dihydroxybiphenyl 1,2-dioxygenase (23DBDO) gene was constructed. The smallest subclone (pNPX9) encoding 23DBDO activity was sequenced and analyzed. The C-terminal domain of 23DBDO from Comamonas sp. SMN4 had five catalytically essential residues and was more highly conserved than the N-terminal domain. Phylogenetic and structural relationships of 23DBDO from Comamonas sp. SMN4 were analyzed.
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