The pap1-independent induction by metal ions of a third gene encoding glutathione S-transferase gene from the fission yeast
- Authors
- Sa, JH; Shin, YH; Lim, HW; Kim, K; Park, EH; Lim, CJ
- Issue Date
- Dec-2002
- Publisher
- SPRINGER-VERLAG SINGAPORE PTE LTD
- Keywords
- glutathione S-Transferase; GST-lacZ; metal ions; Pap1; regulation; Schizosaccharomyces pombe
- Citation
- MOLECULES AND CELLS, v.14, no.3, pp 444 - 448
- Pages
- 5
- Journal Title
- MOLECULES AND CELLS
- Volume
- 14
- Number
- 3
- Start Page
- 444
- End Page
- 448
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16368
- ISSN
- 1016-8478
0219-1032
- Abstract
- A third gene that encodes glutathione S-transferase (GSTIII) was previously cloned from the fission yeast Schizosaccharomyces pombe. Using the GST111-1acZ fusion plasmid pGDA-19, its expression was shown to be enhanced by various metal ions. In the present study, four additional fusion plasmids, pGDA-29, pGDA-39, PGDA-49, and pGDA-59, were designed to carry 998, 378, 276, and 115 bp upstream regions from the translational initiation point, respectively. The major activation region was located between -998 and -378 bp upstream of the GSTIII gene. Regulatory sequences that are responsible for the induction by metal ions reside between -998 and -378 bp and between -276 and -115 bp upstream of the gene. The overexpressed Pap1 exerts a repression effect on the GSTIII expression via -998 similar to -378 bp region, whereas it exerts an activation effect on the GSTIII expression via -270 similar to -115 bp region. However, the induction of the GSTIII gene by metal ions occurs independent of Pap1.
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