Regulation of the manganese-containing superoxide dismutase gene from fission yeast
- Authors
- Jung, HI; Lee, YY; Lim, HW; Ahn, KS; Park, EH; Lim, CJ
- Issue Date
- Oct-2002
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- fission yeast; MnSOD; Schizosaccharomyces pombe; superoxide dismutase; transcriptional regulation
- Citation
- MOLECULES AND CELLS, v.14, no.2, pp 300 - 304
- Pages
- 5
- Journal Title
- MOLECULES AND CELLS
- Volume
- 14
- Number
- 2
- Start Page
- 300
- End Page
- 304
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16385
- ISSN
- 1016-8478
0219-1032
- Abstract
- The manganese superoxide dismutase (MnSOD) is a mitochondrial enzyme that dismutates a potentially toxic superoxide radical into hydrogen peroxide and dioxygen. To study the regulation of the Schizosaccharomyces pombe MnSOD gene, the 943 bp upstream region was fused into the promoterless beta-galactosidase gene of the shuttle vector YEp357, which resulted in the fusion plasmid pMS14. Restriction mapping and nucleotide sequencing confirmed its construction. The synthesis of beta-galactosidase from the fusion plasmid was induced by aluminum chloride, menadione, cadmium chloride, manganese chloride, and hydrogen peroxide. It was also induced by NO-generating S-nitroso-N-acetylpenicillamine (SNAP). However, cupric chloride and zinc chloride did not affect the synthesis of beta-galactosidase from the fusion plasmid. The beta-galactosidase synthesis appeared to be independent of the Pap1 protein. These results suggest that some metals, oxidative stress, and nitric oxide regulate the S. pombe MnSOD gene.
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