Characterization, expression and regulation of a third gene encoding glutathione S-transferase from the fission yeast
- Authors
- Shin, YH; Park, EH; Fuchs, JA; Lim, CJ
- Issue Date
- Aug-2002
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- fission yeast; genomic DNA; glutathione S-transferase; regulation; Schizosaccharomyes pombe
- Citation
- BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, v.1577, no.1, pp 164 - 170
- Pages
- 7
- Journal Title
- BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
- Volume
- 1577
- Number
- 1
- Start Page
- 164
- End Page
- 170
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16402
- DOI
- 10.1016/S0167-4781(02)00422-0
- ISSN
- 0167-4781
- Abstract
- A third gene encoding glutathione S-transferase (GSTIII) was cloned from the fission yeast Schizosaccharomyces pombe. The nucleotide sequence determined was found to contain 2110 base pairs including an open reading frame of 242 amino acids that would encode a protein of a molecular mass of 26,620 Da. The cloned GSTIII gene could be expressed in S. pombe, S. cerevisiae and Escherichia coli cells which gave 1.4-, 2.1-, and 3.0-fold higher GST activity in an assay using 1-chloro-2,4-dinitrobenzene as a substrate, respectively. The cloned GSTIII gene caused higher survivals of S. pombe cells on solid media with cadmium chloride or mercuric chloride. The GSTIII protein has 16% and 18% homologies with the GSTI and GSTII proteins, respectively. To independently monitor the regulation of the GSTIII gene, its 1168 bp upstream region and N-terminal 33 amino acid-coding region was fused into the promoterless p-galactosidase gene of the shuttle vector YEp357. The synthesis of beta-galactosidase from the fusion plasmid pGY357 was greatly enhanced by cadmium chloride (50 M), cupric chloride (10 muM), aluminum chloride (5 mM, 10 mM), mercuric chloride (1 muM), and zinc chloride (10 mM). However, the synthesis of beta-galactosidase from the fusion plasmid pGY357 was not affected by superoxide-generating menadione, and o-dinitrobenzene, whereas they could significantly induce the expression of the GSTI and GSTII genes of S. pombe. The overproduced Pap I inhibited the induction of beta-galactosidase synthesis from the fusion plasmid pGY357 by cadmium chloride, which is opposite to the previously known role of PapI in the response to oxidative stress. Our results collectively indicate that the three GST genes of S. pombe are subjected to different regulatory mechanisms. The major role of the GSTIII protein in S. pombe may be the detoxification of various metals. (C) 2002 Elsevier Science B.V. All rights reserved.
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