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Molecular cloning and expression of nahB gene from Pseudomonas fluorescens in E-coliPseudomonas fluorescens 의 nahB 유전자의 분자적 클로닝과 E. coli 세포내에서의 발현

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Pseudomonas fluorescens 의 nahB 유전자의 분자적 클로닝과 E. coli 세포내에서의 발현
Authors
Whang, KHChoi, SYLee, NRMin, KH
Issue Date
Dec-2001
Publisher
The Genetics society of Korea
Keywords
dihydrodiol naphthalene dehydrogenase; nahB gene; Pseudomonas fluorescens
Citation
KOREAN JOURNAL OF GENETICS, v.23, no.4, pp 377 - 383
Pages
7
Journal Title
KOREAN JOURNAL OF GENETICS
Volume
23
Number
4
Start Page
377
End Page
383
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16634
ISSN
0254-5934
Abstract
A gene encoding the cis-dihydrodiol naphthalene (DFIN) dehydrogenase has been cloned from genomic DNA of Pseudomonas fluorescens SM11. The DNA fragment containing the nahB gene for the cis-DHN dehydrogenase was mapped with restriction endonucleases and sequenced. The DNA fragment contained an ORF of 780 bp encoding a polypeptide of 260 amino acid residues. The amino acid sequences of the enzyme revealed several conserved regions with those of the corresponding enzyme encoded in other strains of Pseudomonas. The homology of the amino acid sequence is 93% identity with that of the enzyme by P. putida OUS82. It is assumed that the cis-DHN dehydrogenase consists of four 28,800 molecular weight subunits and is specific for NAD as an electron acceptor for catalytic function.
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