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Structure and expression of the Arabidopsis thaliana homeobox gene Athb-12

Authors
Lee, YHOh, HSCheon, CIHwang, ITKim, YJChun, JY
Issue Date
Jun-2001
Publisher
ACADEMIC PRESS INC
Keywords
abscisic acid; homeobox; transcriptional activation domain; water stress
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.284, no.1, pp.133 - 141
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume
284
Number
1
Start Page
133
End Page
141
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16651
DOI
10.1006/bbrc.2001.4904
ISSN
0006-291X
Abstract
We have isolated the Arabidopsis thaliana homeobox gene Athb-12, determined its structure and activation domain, demonstrated that its promoter is inducible in response to abscisic acid (ABA) treatment, and characterized the cellular distribution of its transcripts. The single intron of the gene interrupted the leucine-zipper domain region. The 5 ' regulatory region of Athb-12 can drive beta -glucuronidase (GUS) expression in tobacco transgenic plants. Athb-12 gene expression was further examined using in situ hybridization to determine the cellular distribution of Athb-12 transcripts during ABA induction. A complex pattern of Athb-12 expression was observed, often associated with regions of developing vascular tissues. Analysis of chimeras constructed from Athb-12 and the DNA-binding domain of the Saccharomyces cerevisiae transcription factor GAL4 revealed that the activation domain of Athb-12 lies in the C-terminal region (amino acids 180 to 235). Taken together, our data suggest that Athb-12 is a transcriptional activator important in regulating certain developmental processes as well as in the plant's response to water stress involving ABA-mediated gene expression. (C) 2001 Academic Press.
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