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Molecular cloning and analysis of the hisH gene encoding glutamine amidotransferase from Corynebacterium glutamicum

Authors
Kim, JYLee, MS
Issue Date
Jun-2001
Publisher
GENETICS SOC KOREA
Keywords
Corynebacterium glutamicum; glutamine amidotransferase; hisH gene complementation analysis
Citation
KOREAN JOURNAL OF GENETICS, v.23, no.2, pp 121 - 127
Pages
7
Journal Title
KOREAN JOURNAL OF GENETICS
Volume
23
Number
2
Start Page
121
End Page
127
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16655
ISSN
0254-5934
Abstract
The hisH gene of Corynebacterium glutamicum encoding glutamine amidotransferase has been isolated by complementation of his auxotrophic mutant of Escherichia coli, using a C. glutamicum genomic DNA library. Cloned DNA fragments containing hisH gene can also complement hisA, hisF, and hisI auxotrophs of E. coli, suggesting that these genes are clustered in the genome. We determined the nucleotide sequences of the minimal fragment containing the hisH gene region. The gene consists of 636 bp and codes for a polypeptide of 212 amino acids with a molecular mass of about 24 kDa, showing high level of identity with reported amino acid sequences of glutamine amidotransferase from other organisms.
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