Detailed Information

Cited 0 time in webofscience Cited 2 time in scopus
Metadata Downloads

In vitro cytotoxic effect of N-(phosphonacetyl)-L-aspartic acid in liposome against C-26 murine colon carcinoma

Authors
Kim, JSHeath, TD
Issue Date
Apr-2000
Publisher
PHARMACEUTICAL SOCIETY KOREA
Keywords
liposomes; C-26 murine colon carcinoma; PALA; cytotoxicity
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.23, no.2, pp.167 - 171
Journal Title
ARCHIVES OF PHARMACAL RESEARCH
Volume
23
Number
2
Start Page
167
End Page
171
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16774
DOI
10.1007/BF02975508
ISSN
0253-6269
Abstract
We have investigated the in vitro cytotoxic effect of liposome-encapsulated N-(phosphonacetyl)-L-aspartic acid (PALA) against C-26 murine colon cancer cells, and have compared it in this regard to free PALA. Three different PALA-containing liposomal formulations using distearoylphosphatidylcholine (DSPC), distearoylphosphatidylglycerol (DSPG), and polyethyleneglycol-derivatized distearoylphosphatidylethanolamine (PEG-DSPE) were made and their cytotoxicity was measured. In 72 hr continuous exposure experiment with C-26 cells, the 50% growth inhibitory concentration (IC50) of DSPG-PALA liposome formulation was 0.09 mu M, which showed about 65-fold more potent than unencapsulated free PALA (5.1 mu M). Similar degree of increase in cytotoxicity was also observed in 1 hr exposure experiment. However, the IC50 of PEG-DSPE-PALA liposome and DSPC-PALA liposome were 10.7 mu M and 11.8 mu M, respectively, which showed slightly less potent than unencapsulated free PALA. Physical characteristics of PALA-liposomes, such as the size and drug:lipid ratio were also determined. In conclusion, negatively-charged DSPG-PALA liposome showed the highest cytotoxic effect among tested on the C-26 cells in vitro.
Files in This Item
There are no files associated with this item.
Appears in
Collections
약학대학 > 약학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Jin Seok photo

Kim, Jin Seok
약학대학 (약학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE