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Isolation and analysis of the aroE gene encoding shikimate dehydrogenase from Corynebacterium glutamicum

Authors
Yang, HELee, MS
Issue Date
Dec-1999
Publisher
GENETICS SOC KOREA
Keywords
aroE gene; complementation analysis; Corynebacterium glutamicum; shikimate dehydrogenase
Citation
KOREAN JOURNAL OF GENETICS, v.21, no.4, pp.275 - 283
Journal Title
KOREAN JOURNAL OF GENETICS
Volume
21
Number
4
Start Page
275
End Page
283
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16812
ISSN
0254-5934
Abstract
The aroE gene was cloned from Corynebacterium glutamicum by complementation of an Escherichia coli aromatic amino acid auxotroph. The E. coli aroE gene codes for shikimate dehydrogenase, an enzyme involved in the aromatic amino acid biosynthetic pathway. The cloned DNA fragment in the recombinant plasmid, named pROE1, was physically mapped with several restriction endonucleases. We determined the DNA nucleotide sequences of the DNA fragment and reported here the sequences of the aroE gene. The complete nucleotide sequences of the aroE gene showed an open reading frame encoding a protein of 274 amino acids. Comparison of this deduced amino acid sequences of C. glutamicum aroE gene with those of the enzyme from Mycobacterium tuberculosis, Archaeoglobos folgidus and Synechocystis sp. revealed 45%, 32% and 28% similarity, respectively.
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