A second thioltransferase of Schizosaccharomyces pombe contains glutathione S-transferase activity
DC Field | Value | Language |
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dc.contributor.author | Kim, HG | - |
dc.contributor.author | Park, EH | - |
dc.contributor.author | Lim, CJ | - |
dc.date.available | 2021-02-22T16:47:50Z | - |
dc.date.issued | 1999-11 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16814 | - |
dc.description.abstract | Two types of the thioltransferase (also called glutaredoxin) have been previously detected in the cytosolic extract of Schizosaccharomyces pombe, a fission yeast. Previously, the one with a smaller molecular mass (14 kDa) was purified and characterized. In the present study, the second thioltransferase was purified. The purification procedure included ammonium sulfate fractionation (40-80%), Sephadex G-200 gel filtration, DEAE-cellulose ion-exchange chromatography, Sephadex G-50 gel filtration, and glutathione-agarose affinity chromatography. The purified enzyme showed a single band on SDS-PAGE, and its molecular mass was determined to be 23 kDa, It utilizes various compounds as substrates, including 2-hydroxyethyl disulfide. Interestingly, we found that the purified thioltransferase also contains significant glutathione S-transferase activity. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER SINGAPORE PTE LTD | - |
dc.title | A second thioltransferase of Schizosaccharomyces pombe contains glutathione S-transferase activity | - |
dc.type | Article | - |
dc.publisher.location | 싱가폴 | - |
dc.identifier.scopusid | 2-s2.0-0001931013 | - |
dc.identifier.wosid | 000083911400003 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.32, no.6, pp 535 - 540 | - |
dc.citation.title | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | - |
dc.citation.volume | 32 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 535 | - |
dc.citation.endPage | 540 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | PIG-LIVER THIOLTRANSFERASE | - |
dc.subject.keywordPlus | AMINO-ACID-SEQUENCE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GLUTAREDOXIN | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | REDUCTASE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | MUTANT | - |
dc.subject.keywordAuthor | glutaredoxin | - |
dc.subject.keywordAuthor | glutathione S-transferase | - |
dc.subject.keywordAuthor | Schizosaccharomyces pombe | - |
dc.subject.keywordAuthor | thioltransferase | - |
dc.identifier.url | https://kiss.kstudy.com/Detail/Ar?key=427499 | - |
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