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Cloning and sequencing of the fcbB gene encoding 4-chlorobenzoate-coenzyme A dehalogenase from Pseudomonas sp. DJ-12

Authors
Chae, JCKim, YMin, KHKim, YCKim, CK
Issue Date
30-Apr-1999
Publisher
SPRINGER-VERLAG SINGAPORE PTE LTD
Keywords
4-chlorobenzoate-coenzyme A dehalogenase; dechlorination; Pseudomonas sp. DJ-12
Citation
MOLECULES AND CELLS, v.9, no.2, pp.225 - 229
Journal Title
MOLECULES AND CELLS
Volume
9
Number
2
Start Page
225
End Page
229
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16846
ISSN
1016-8478
Abstract
Pseudomonas sp, DJ-12 degrades 4-chlorobenzoate through hydrolytic dechlorination to produce 4-hydroxybenzoate and a chloride ion. The fcbB gene encoding the 4-chlorobenzoate-coenzyme A (4CBA-CoA) dehalogenase which catalyzes the nucleophilic substitution reaction to convert 4CBA-CoA to 4-hydroxybenzoate-coenzyme A (4HBA-CoA) in the consecutive steps of dechlorination was cloned from the chromosome of the organism. A nucleotide sequence analysis of the gene showed an open reading frame consisting of 810 nucleotides, which can encode for a polypeptide of molecular mass 30 kDa, containing 269 amino acid residues. A promoter-like sequence (-35 and -10 region) and a putative ribosome-binding sequence were identified. A deduced amino acid sequence of the 4CBA-CoA dehalogenase showed 86%, 50%, and 50% identity with those of corresponding enzymes in the Pseudomonas sp, CBS3, Arthrobacter sp, SU, and Arthrobacter sp. TM1, respectively.
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