Cloning and sequencing of the fcbB gene encoding 4-chlorobenzoate-coenzyme A dehalogenase from Pseudomonas sp. DJ-12
DC Field | Value | Language |
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dc.contributor.author | Chae, JC | - |
dc.contributor.author | Kim, Y | - |
dc.contributor.author | Min, KH | - |
dc.contributor.author | Kim, YC | - |
dc.contributor.author | Kim, CK | - |
dc.date.available | 2021-02-22T16:48:16Z | - |
dc.date.issued | 1999-04 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16846 | - |
dc.description.abstract | Pseudomonas sp, DJ-12 degrades 4-chlorobenzoate through hydrolytic dechlorination to produce 4-hydroxybenzoate and a chloride ion. The fcbB gene encoding the 4-chlorobenzoate-coenzyme A (4CBA-CoA) dehalogenase which catalyzes the nucleophilic substitution reaction to convert 4CBA-CoA to 4-hydroxybenzoate-coenzyme A (4HBA-CoA) in the consecutive steps of dechlorination was cloned from the chromosome of the organism. A nucleotide sequence analysis of the gene showed an open reading frame consisting of 810 nucleotides, which can encode for a polypeptide of molecular mass 30 kDa, containing 269 amino acid residues. A promoter-like sequence (-35 and -10 region) and a putative ribosome-binding sequence were identified. A deduced amino acid sequence of the 4CBA-CoA dehalogenase showed 86%, 50%, and 50% identity with those of corresponding enzymes in the Pseudomonas sp, CBS3, Arthrobacter sp, SU, and Arthrobacter sp. TM1, respectively. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER-VERLAG SINGAPORE PTE LTD | - |
dc.title | Cloning and sequencing of the fcbB gene encoding 4-chlorobenzoate-coenzyme A dehalogenase from Pseudomonas sp. DJ-12 | - |
dc.type | Article | - |
dc.publisher.location | 싱가폴 | - |
dc.identifier.scopusid | 2-s2.0-0033616954 | - |
dc.identifier.wosid | 000080151400017 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.9, no.2, pp 225 - 229 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 9 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 225 | - |
dc.citation.endPage | 229 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | 4-CHLOROBENZOYL COENZYME-A | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SP CBS3 | - |
dc.subject.keywordPlus | DECHLORINATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | 4-chlorobenzoate-coenzyme A dehalogenase | - |
dc.subject.keywordAuthor | dechlorination | - |
dc.subject.keywordAuthor | Pseudomonas sp. DJ-12 | - |
dc.identifier.url | https://www.molcells.org/journal/view.html?pn=search&uid=3137&vmd=Full | - |
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