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Effect of NaCl on Thermal Resistance, Antibiotic Resistance, and Human Epithelial Cell Invasion of Listeria monocytogenes

Authors
Lee, JinheeYoon, HyunjooLee, SunahYoon, Yohan
Issue Date
Oct-2012
Publisher
KOREAN SOC FOOD SCIENCE ANIMAL RESOURCES
Keywords
Listeria monocytogenes; NaCl; heat resistance; antibiotics; Caco-2 cell line
Citation
KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES, v.32, no.5, pp.545 - 552
Journal Title
KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES
Volume
32
Number
5
Start Page
545
End Page
552
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/17512
DOI
10.5851/kosfa.2012.32.5.545
ISSN
1225-8563
Abstract
This study evaluated the effects of NaCl on heat resistance and Caco-2 cell invasion of Listeria monocytogenes in broth media and sausage. A 10-strain mixture of L. monocytogenes was inoculated in tryptic soy broth containing 0.6% yeast extract (TSBYE), and sausage formulated with 0, 2, 4, and 6% NaCl. The medium was stored at 7, 15, 20, and 25 degrees C for 3-16 d, and medium samples were withdrawn at the appropriate time and challenged to 55, 60, and 63 degrees C to evaluate the thermal resistance of the pathogen. Sausage samples were stored at 7 and 25 degrees C, and they were exposed to 63 degrees C to evaluate thermal resistance. NaCl-habituated L. inonocytogenes strains NCCP10811 and NCCP10943 were examined for 12 antibiotics and Caco-2 cell invasion assay (only L. monocytogenes NCCP10943). Bacterial populations of L. monocytogenes generally increased (p<0.05) during the heat challenge as NaCl concentrations increased in both TSBYE and sausage samples. The antibiotic resistance of L. monocytogenes was not observed (p >= 0.05) when it was exposed to a single concentration of NaCl in TSBYE, but the pathogen obtained resistance to some antibiotics when exposed to a sequential increase of NaCl concentration. Invasion efficiency of L. monocytogenes NCCP10943 was not increased (p >= 0.05) with NaCl concentration increase. These results indicate that NaCl may increase the resistance of L. monocytogenes to heat and to some antibiotics, but may not increase Caco-2 cell invasion of L. monocytogenes.
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