Effect of NaCl on Thermal Resistance, Antibiotic Resistance, and Human Epithelial Cell Invasion of Listeria monocytogenes
- Authors
- Lee, Jinhee; Yoon, Hyunjoo; Lee, Sunah; Yoon, Yohan
- Issue Date
- Oct-2012
- Publisher
- KOREAN SOC FOOD SCIENCE ANIMAL RESOURCES
- Keywords
- Listeria monocytogenes; NaCl; heat resistance; antibiotics; Caco-2 cell line
- Citation
- KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES, v.32, no.5, pp 545 - 552
- Pages
- 8
- Journal Title
- KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES
- Volume
- 32
- Number
- 5
- Start Page
- 545
- End Page
- 552
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/17513
- DOI
- 10.5851/kosfa.2012.32.5.545
- ISSN
- 1225-8563
- Abstract
- This study evaluated the effects of NaCl on heat resistance and Caco-2 cell invasion of Listeria monocytogenes in broth media and sausage. A 10-strain mixture of L. monocytogenes was inoculated in tryptic soy broth containing 0.6% yeast extract (TSBYE), and sausage formulated with 0, 2, 4, and 6% NaCl. The medium was stored at 7, 15, 20, and 25 degrees C for 3-16 d, and medium samples were withdrawn at the appropriate time and challenged to 55, 60, and 63 degrees C to evaluate the thermal resistance of the pathogen. Sausage samples were stored at 7 and 25 degrees C, and they were exposed to 63 degrees C to evaluate thermal resistance. NaCl-habituated L. inonocytogenes strains NCCP10811 and NCCP10943 were examined for 12 antibiotics and Caco-2 cell invasion assay (only L. monocytogenes NCCP10943). Bacterial populations of L. monocytogenes generally increased (p<0.05) during the heat challenge as NaCl concentrations increased in both TSBYE and sausage samples. The antibiotic resistance of L. monocytogenes was not observed (p >= 0.05) when it was exposed to a single concentration of NaCl in TSBYE, but the pathogen obtained resistance to some antibiotics when exposed to a sequential increase of NaCl concentration. Invasion efficiency of L. monocytogenes NCCP10943 was not increased (p >= 0.05) with NaCl concentration increase. These results indicate that NaCl may increase the resistance of L. monocytogenes to heat and to some antibiotics, but may not increase Caco-2 cell invasion of L. monocytogenes.
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