Synthesis of nitrogen-doped carbon nanodots to destroy bacteria competing with Campylobacter jejuni in enrichment medium, and development of a monoclonal antibody to detect C. jejuni after enrichment
- Authors
- Ha, Jimyeong; Seo, Yeongeun; Kim, Yujin; Lee, Jeeyeon; Lee, Heeyoung; Kim, Sejeong; Choi, Yukyung; Oh, Hyemin; Lee, Yewon; Park, Eunyoung; Kang, Joohyun; Yoon, Yohan
- Issue Date
- Feb-2021
- Publisher
- Elsevier B.V.
- Keywords
- Campylobacter jejuni; Detection method; Light emitting diode; Monoclonal antibody; Nanodots
- Citation
- International Journal of Food Microbiology, v.339, pp 1 - 7
- Pages
- 7
- Journal Title
- International Journal of Food Microbiology
- Volume
- 339
- Start Page
- 1
- End Page
- 7
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/17733
- DOI
- 10.1016/j.ijfoodmicro.2020.109014
- ISSN
- 0168-1605
1879-3460
- Abstract
- The objective of this study was to develop a method with improved sensitivity for Campylobacter jejuni detection in foods. Nitrogen-doped carbon nanodots (N-CNDs) were synthesized and added to an enrichment medium (Bolton broth) at a concentration of 10 mg/mL. A light-emitting diode (LED) at a wavelength of 425 nm was used to irradiate the N-CNDs-supplemented enrichment medium to induce an exothermic reaction for 1 h. Additionally, a monoclonal antibody specific to C. jejuni NCTC11168 was developed using hybridoma cells to aid detection. The C. jejuni detection capabilities of N-CNDs-supplemented enrichment medium and the conventional Bolton broth enrichment, were compared using duck samples. C. jejuni in the enrichment was detected with the monoclonal antibody based-indirect enzyme-linked immunosorbent assay (ID-ELISA). The N-CNDs-supplemented enrichment medium showed a better C. jejuni detection capability than the conventional Bolton broth enrichment. Additionally, data from ID-ELISA showed excellent detection efficiency and a shortened detection time in the N-CNDs-supplemented enrichment medium after LED irradiation at 425 nm. These results indicate that 1-h LED irradiation at 425 nm to Bolton broth supplemented with the N-CNDs increased the detection efficiency and shortened the detection time with the monoclonal antibody for C. jejuni in food. © 2020 Elsevier B.V.
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