Unique Triterpenoid of Jujube Root Protects Cisplatin-induced Damage in Kidney Epithelial LLC-PK1 Cells via Autophagy Regulationopen access
- Authors
- Lee, Dahae; Kang, Kyo Bin; Kim, Hyun Woo; Park, Jung Sik; Hwang, Gwi Seo; Kang, Ki Sung; Choi, Sungyoul; Yamabe, Noriko; Kim, Ki Hyun
- Issue Date
- Mar-2020
- Publisher
- MDPI
- Keywords
- cisplatin; nephrotoxicity; autophagy; Ziziphus jujuba
- Citation
- NUTRIENTS, v.12, no.3
- Journal Title
- NUTRIENTS
- Volume
- 12
- Number
- 3
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/2499
- DOI
- 10.3390/nu12030677
- ISSN
- 2072-6643
- Abstract
- Chronic exposure to cisplatin is associated with irreversible kidney impairment. In this present study, we explored the protective effects of 3-dehydroxyceanothetric acid 2-methyl ester (3DC2ME) isolated from roots of jujube (Ziziphus jujuba, Rhamnaceae) against cisplatin-induced damage in vitro. In kidney epithelial LLC-PK1 cells, western blotting and staining with specific autophagy epifluorescent dye CytoID were used to determine the molecular pathways involving autophagy. Treatment with 3DC2ME reduced the increased Cyto-ID-stained autophagic vesicles and reversed the protein expressions of 5' AMP-activated protein kinase subunit beta-1 (AMPK)/mammalian target of rapamycin (mTOR)-dependent signaling pathway in cisplatin-induced cell death. Additionally, treatment with autophagy inhibitor 3-methyladenine (3-MA) and with or without 3DC2ME attenuated the cisplatin-induced apoptosis. Although further research is necessary to substantiate the effects, we evaluated the potential mechanism of action of 3DC2ME as an adjuvant for cancer patients.
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