Detailed Information

Cited 0 time in webofscience Cited 2 time in scopus
Metadata Downloads

Unique Triterpenoid of Jujube Root Protects Cisplatin-induced Damage in Kidney Epithelial LLC-PK1 Cells via Autophagy Regulationopen access

Authors
Lee, DahaeKang, Kyo BinKim, Hyun WooPark, Jung SikHwang, Gwi SeoKang, Ki SungChoi, SungyoulYamabe, NorikoKim, Ki Hyun
Issue Date
Mar-2020
Publisher
MDPI
Keywords
cisplatin; nephrotoxicity; autophagy; Ziziphus jujuba
Citation
NUTRIENTS, v.12, no.3
Journal Title
NUTRIENTS
Volume
12
Number
3
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/2499
DOI
10.3390/nu12030677
ISSN
2072-6643
Abstract
Chronic exposure to cisplatin is associated with irreversible kidney impairment. In this present study, we explored the protective effects of 3-dehydroxyceanothetric acid 2-methyl ester (3DC2ME) isolated from roots of jujube (Ziziphus jujuba, Rhamnaceae) against cisplatin-induced damage in vitro. In kidney epithelial LLC-PK1 cells, western blotting and staining with specific autophagy epifluorescent dye CytoID were used to determine the molecular pathways involving autophagy. Treatment with 3DC2ME reduced the increased Cyto-ID-stained autophagic vesicles and reversed the protein expressions of 5' AMP-activated protein kinase subunit beta-1 (AMPK)/mammalian target of rapamycin (mTOR)-dependent signaling pathway in cisplatin-induced cell death. Additionally, treatment with autophagy inhibitor 3-methyladenine (3-MA) and with or without 3DC2ME attenuated the cisplatin-induced apoptosis. Although further research is necessary to substantiate the effects, we evaluated the potential mechanism of action of 3DC2ME as an adjuvant for cancer patients.
Files in This Item
Appears in
Collections
약학대학 > 약학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kang, Kyo Bin photo

Kang, Kyo Bin
약학대학 (약학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE