Biodiesel and flavor compound production using a novel promiscuous cold-adapted SGNH-type lipase (HaSGNH1) from the psychrophilic bacterium Halocynthiibacter arcticusopen access
- Authors
- Le, Ly Thi Huong Luu; Yoo, Wanki; Jeon, Sangeun; Lee, Changwoo; Kim, Kyeong Kyu; Lee, Jun Hyuck; Kim, T. Doohun
- Issue Date
- Mar-2020
- Publisher
- BioMed Central
- Keywords
- HaSGNH1; Halocynthiibacter arcticus; Immobilization; SGNH-type lipase; Substrate specificity; Biodiesel
- Citation
- Biotechnology for Biofuels, v.13, no.1
- Journal Title
- Biotechnology for Biofuels
- Volume
- 13
- Number
- 1
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/2503
- DOI
- 10.1186/s13068-020-01696-x
- ISSN
- 1754-6834
1754-6834
- Abstract
- Background Biodiesel and flavor compound production using enzymatic transesterification by microbial lipases provides mild reaction conditions and low energy cost compared to the chemical process. SGNH-type lipases are very effective catalysts for enzymatic transesterification due to their high reaction rate, great stability, relatively small size for convenient genetic manipulations, and ease of immobilization. Hence, it is highly important to identify novel SGNH-type lipases with high catalytic efficiencies and good stabilities. Results A promiscuous cold-adapted SGNH-type lipase (HaSGNH1) from Halocynthiibacter arcticus was catalytically characterized and functionally explored. HaSGNH1 displayed broad substrate specificity that included tert-butyl acetate, glucose pentaacetate, and p-nitrophenyl esters with excellent stability and high efficiency. Important amino acids (N83, M86, R87, F131, and I173F) around the substrate-binding pocket were shown to be responsible for catalytic activity, substrate specificity, and reaction kinetics. Moreover, immobilized HaSGNH1 was used to produce high yields of butyl and oleic esters. Conclusions This work provides a molecular understanding of substrate specificities, catalytic regulation, immobilization, and industrial applications of a promiscuous cold-adapted SGNH-type lipase (HaSGNH1) from H. arcticus. This is the first analysis on biodiesel and flavor synthesis using a cold-adapted halophilic SGNH-type lipase from a Halocynthiibacter species.
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