Deficiency of calpain-6 inhibits primary ciliogenesisopen access
- Authors
- Kim, Bo Hye; Kim, Do Yeon; Oh, Sumin; Ko, Je Yeong; Rah, Gyuyeong; Yoo, Kyung Hyun; Park, Jong Hoon
- Issue Date
- Oct-2019
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Keywords
- Acetylated alpha-tubulin; Calpain-6; mRNA microarray; Primary cilia; Tubulin code
- Citation
- BMB REPORTS, v.52, no.10, pp 619 - 624
- Pages
- 6
- Journal Title
- BMB REPORTS
- Volume
- 52
- Number
- 10
- Start Page
- 619
- End Page
- 624
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/2812
- DOI
- 10.5483/BMBRep.2019.52.10.182
- ISSN
- 1976-6696
1976-670X
- Abstract
- The primary cilium is a microtubule-based structure projecting from a cell. Although the primary cilium shows no motility, it can recognize environmental stimuli. Thus, ciliary defects cause severe abnormalities called ciliopathies. Ciliogenesis is a very complex process and involves a myriad of components and regulators. In order to excavate the novel positive regulators of ciliogenesis, we performed mRNA microarray using starved NIH/3T3 cells. We selected 62 murine genes with corresponding human orthologs, with significantly upregulated expression at 24 h after serum withdrawal. Finally, calpain-6 was selected as a positive regulator of ciliogenesis. We found that calpain-6 deficiency reduced the percentage of ciliated cells and impaired sonic hedgehog signaling. It has been speculated that this defect might be associated with decreased levels of.-tubulin acetylation at lysine 40. This is the first study to report a novel role of calpain-6 in the formation of primary cilia.
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