Microfluidic Generation of Amino-Functionalized Hydrogel Microbeads Capable of On-Bead Bioassayopen access
- Authors
- Kim, Seongsoo; Lee, Sang-Myung; Lee, Sung Sik; Shin, Dong-Sik
- Issue Date
- Aug-2019
- Publisher
- MDPI
- Keywords
- polyethylene glycol; hydrogel microbead; microfluidic channel; on-bead bioassay; peptide synthesis
- Citation
- MICROMACHINES, v.10, no.8
- Journal Title
- MICROMACHINES
- Volume
- 10
- Number
- 8
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/2910
- DOI
- 10.3390/mi10080527
- ISSN
- 2072-666X
2072-666X
- Abstract
- Microfluidic generation of hydrogel microbeads is a highly efficient and reproducible approach to create various functional hydrogel beads. Here, we report a method to prepare crosslinked amino-functionalized polyethylene glycol (PEG) microbeads using a microfluidic channel. The microbeads generated from a microfluidic device were evaluated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and confocal laser scanning microscopy, respectively. We found that the microbeads were monodisperse and the amino groups were localized on the shell region of the microbeads. A swelling test exhibited compatibility with various solvents. A cell binding assay was successfully performed with RGD peptide-coupled amino-functionalized hydrogel microbeads. This strategy will enable the large production of the various functional microbeads, which can be used for solid phase peptide synthesis and on-bead bioassays.
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