축·수산·전통식품 중 황색포도상 구균의 정량적 분석을 위한 Petrifilm TM Staph Express Count Plate의 성능 평가Efficiency of Petrifilm TM Staph Express Count Plate for the Enumeration of Staphylococcus aureus in Meat, Fishery Product, and Korean Traditional Foods
- Other Titles
- Efficiency of Petrifilm TM Staph Express Count Plate for the Enumeration of Staphylococcus aureus in Meat, Fishery Product, and Korean Traditional Foods
- Authors
- 유윤정; 최유나; 최승호; 방현조; 윤요한; 하지명; 이수민
- Issue Date
- Jun-2019
- Publisher
- 한국식품위생안전성학회
- Keywords
- 3M TM Petrifilm TM Staph Express Count Plate; Baird-Parker Agar; Staphylococcus aureus; Selective media; Detection
- Citation
- 한국식품위생안전성학회지, v.34, no.3, pp 309 - 315
- Pages
- 7
- Journal Title
- 한국식품위생안전성학회지
- Volume
- 34
- Number
- 3
- Start Page
- 309
- End Page
- 315
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/2983
- DOI
- 10.13103/JFHS.2019.34.3.309
- ISSN
- 1229-1153
2465-9223
- Abstract
- To enumerate Staphylococcus aureus in food, Baird-Parker Agar (BPA) is usually used in the conventional method, However it requires time and space for the preparation and plating, and incubation. Thus, use of the 3M TM Petrifilm TM Staph Express Count Plate (STX Petrifilm) might be appropriate to solve these challenging problems. The purpose of this study was to compare the efficiency of STX Petrifilm with BPA for enumeration of S. aureus in various foods. A mixture of S. aureus strains ATCC29213, ATCC25923, and ATCC13565 was inoculated on marinated pork chop, beef (chuck tender), dried filefish, semi-dried squid, rice cake, and Japchae (stir-fried glass noodles) at 2, 3, 5, and 7 Log CFU/g. S. aureus cell counts were enumerated by spread-plating on STX Petrifilm and BPA after 0 and 24 hours at 4 o C (marinated pork chop, beef, semi-dried squid, and stir-fried glass noodles) and 25 o C (dried filefish and rice cake). Recovery of STX Petrifilm for S. aureus from various food samples was compared with BPA, and the results showed that there were no significant differences between two selective media in all cases. The results indicated that STX Petrifilm had enough efficiency to recover S. aureus from various foods as well as saving time and space.
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