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NaCl Effect on Invasive Staphylococcus aureus Invasion and Immune Responses in the Intestinal Epithelial Cell

Authors
윤요한하지명이수민김세정이지연최유경오혜민최경희
Issue Date
Apr-2019
Publisher
Austin Publishing Group
Citation
Journal of Bacteriology and Mycology, v.6, no.3, pp.1 - 7
Journal Title
Journal of Bacteriology and Mycology
Volume
6
Number
3
Start Page
1
End Page
7
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/3665
ISSN
2471-0172
Abstract
This study investigated the immune responses, which may cause chronic problems through intestinal invasion by Staphylococcus aureus, which is wellknown to cause intoxication rather than invasion, and NaCl effect on the invasion. Five S. aureus strains were cultured and exposed to NaCl concentrations of 0%, 2%, 4%, or 6% supplemented in tryptic soy broth. The surviving cells were isolated by subsequent exposure of the culture on tryptic soy agar containing 0%, 2%, 4%, or 6% NaCl. The resulting cells were subjected to assays evaluating the invasion efficiency into Caco-2 cells, the level of immune responses generated, and the resultant cell viability. For S. aureus strains that invaded Caco-2 cells, the transcriptional analysis for adhesion related genes was conducted by quantitative real-time PCR. Only S. aureus ATCC14458 demonstrated obvious invasion into Caco-2 cells. Invasion efficiency of the strain was influenced by NaCl levels, and expression levels of the cell adhesion related genes were higher at 2%, 4%, and 6% NaCl. In addition, inflammation-related factors were increased. These results indicate that S. aureus ATCC14458 can invade Caco- 2 cells, which may cause infectious illness and other chronic symptoms, and that NaCl increases the extent of Caco-2 cell invasion by expressing specific genes.
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생활과학대학 (식품영양학과)
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