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Production of stearidonic acid-rich triacylglycerol via a two-step enzymatic esterification

Authors
Kim, Nam HoKim, HeejinChoi, NakyungKim, YanghaKim, Byung HeeKim, In-Hwan
Issue Date
Jan-2019
Publisher
ELSEVIER SCI LTD
Keywords
Esterification; Lipase; Lipozyme TL IM; Novozym 435; Stearidonic acid; Triacylglycerol
Citation
FOOD CHEMISTRY, v.270, pp 332 - 337
Pages
6
Journal Title
FOOD CHEMISTRY
Volume
270
Start Page
332
End Page
337
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/3857
DOI
10.1016/j.foodchem.2018.07.121
ISSN
0308-8146
1873-7072
Abstract
The aim of this study was to synthesize stearidonic acid (SDA)-rich triacylglycerol (TAG) via a two-step lipase-catalyzed esterification under vacuum. SDA-rich fatty acid, which was prepared from echium oil via Candida rugosa lipase-catalyzed selective esterification, was used as the substrate. Two different immobilized lipases, Novozym 435 from Candida antarctica and Lipozyme TL IM from Thermomyces lanuginosus, were employed for the synthesis of SDA-rich TAG. In the first step, Novozym 435-catalyzed esterification of the SDA-rich fatty acid with glycerol was carried out for 2 h. In the second step, Lipozyme TL IM-catalyzed esterification of the reaction mixture from the first step was performed for an additional 10 h. The optimal reaction conditions for the second step were a temperature of 65 degrees C, an enzyme loading of 20%, and a vacuum of 0.7 kPa. Consequently, the maximum TAG conversion of ca. 86.4 wt% was obtained after 12 h via a two-step lipase-catalyzed esterification.
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Kim, Byung Hee
생활과학대학 (식품영양학과)
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