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MPN-PCR 방법을 이용한 Vibrio vulnificus 균수 정량분석Quantitative Cell Count of Vibrio vulnificus Cells Based on MPN-PCR Method

Other Titles
Quantitative Cell Count of Vibrio vulnificus Cells Based on MPN-PCR Method
Authors
장유미박슬기정희진이장원윤요한박권삼신일식김영목
Issue Date
Oct-2018
Publisher
한국식품위생안전성학회
Keywords
Injured cell; MPN-PCR; Quantitative cell count; Vibrio vulnificus
Citation
한국식품위생안전성학회지, v.33, no.5, pp.412 - 415
Journal Title
한국식품위생안전성학회지
Volume
33
Number
5
Start Page
412
End Page
415
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/4226
DOI
10.13103/JFHS.2018.33.5.412
ISSN
1229-1153
Abstract
The objective of this study was to establish a quantitative count method of Vibrio vulnificus cells. Plate count method is often used to count the number of V. vulnificus cell using thiosulfate citrate bile salts sucrose (TCBS) agar plate. However, this method is unsuitable for counting V. vulnificus cells due to growth inhibition and cell injuries in TCBS medium. In this study, we suggested a most probable number-polymerase chain reaction (MPN-PCR) method using alkaline peptone water medium for quantification of V. vulnificus. This MPN-PCR method showed 2 log higher cell number than TCBS agar plate method. Similar result were also found in the control using, Luria-Bertani agar containing 2% NaCl. Thus, this MPN-PCR method can be used an sensitive method for quantitative count of V. vulnificus cells in fish and shellfish samples.
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생활과학대학 (식품영양학과)
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