Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Combination of Hydrophobic Filtration and Enrichment Methods for Detecting Bacillus cereus in Fresh-Cut CabbageCombination of Hydrophobic Filtration and Enrichment Methods for Detecting Bacillus cereus in Fresh-Cut Cabbage

Other Titles
Combination of Hydrophobic Filtration and Enrichment Methods for Detecting Bacillus cereus in Fresh-Cut Cabbage
Authors
이수정최유경이희영김세정이지연하지명오혜민이예원김유진윤요한이수민
Issue Date
Oct-2018
Publisher
한국식품위생안전성학회
Keywords
Bacillus cereus; Fresh-cut cabbage; Multiplex PCR; Enrichment; Hydrophobic filtration
Citation
한국식품위생안전성학회지, v.33, no.5, pp.325 - 329
Journal Title
한국식품위생안전성학회지
Volume
33
Number
5
Start Page
325
End Page
329
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/4227
DOI
10.13103/JFHS.2018.33.5.325
ISSN
1229-1153
Abstract
This study developed a rapid detection method for Bacillus cereus in fresh-cut cabbages. Fresh-cut cabbage samples were inoculated at 1-, 2-, and 3-Log CFU/g, and the pathogens were enriched in tryptic soy broth containing 0.15% polymyxin B at 30, 37, and 42oC to determine detection limit and appropriate enrichment temperature for multiplex PCR detection. Enriched bacterial cells in enrichment broth were collected in a hydrophobic filter prior to DNA extraction for multiplex PCR. Filters were resuspended in distilled water, and DNA was extracted from the suspension. DNA samples were further analyzed by multiplex PCR. Detection limit of the multiplex PCR was 5-Log CFU/mL. B. cereus cell counts were higher (p < 0.05) at 42oC than other temperatures. Detection rate of l-, 2-, and 3-Log CFU/g inoculated samples were 60, 80, and 100 % after enrichment respectively. However, when enriched samples were filtered with hydrophobic membrane filter, detection rates became 100%, regardless of inoculation level. Results indicate a combination of enrichment with hydrophobic filtration improves rapid detection efficiency of B. cereus in fresh-cut cabbage by multiplex PCR.
Files in This Item
Go to Link
Appears in
Collections
생활과학대학 > 식품영양학과 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Yoon, Yo Han photo

Yoon, Yo Han
생활과학대학 (식품영양학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE