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A colorimetric hydrogel biosensor for rapid detection of nitrite ions

Authors
Nam, JihyeJung, In-BokKim, BoyoonLee, Sang-MyungKim, Seong-EunLee, Kook-NyungShin, Dong-Sik
Issue Date
Oct-2018
Publisher
ELSEVIER SCIENCE SA
Keywords
Nitrite ions; Rapid detection; Griess assay; Glass fiber; Hydrogel
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v.270, pp 112 - 118
Pages
7
Journal Title
SENSORS AND ACTUATORS B-CHEMICAL
Volume
270
Start Page
112
End Page
118
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/4238
DOI
10.1016/j.snb.2018.04.171
ISSN
0925-4005
Abstract
Nitrite ions are important markers of inflammation, food safety, and water quality; therefore, various methods are under development to detect nitrite ions. This paper describes a novel method for detecting nitrite ions using polyethylene glycol (PEG) hydrogel superimposed glass fiber membrane strips. A colorimetric Griess assay was performed on the hydrogel modified with N-(1-naphthyl)ethylenediamine by dropping a solution of nitrite and sulfanilamide. This colorimetric hydrogel sensor exhibited a detection limit as low as 10 mu M for nitrite ions and the working concentration of nitrite ions was between 10 mu M and 5 mM. The colorimetric reaction was completed within a few seconds; the performance test results indicated that the sensor was specific to nitrite ions compared to other anions, and it was suitable to detect nitrite ions in clinical samples. In the future, this sensor platform can be used to detect nitrite ions in various complex solutions including biological and environmental samples. (C) 2018 Elsevier B.V. All rights reserved.
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공과대학 (화공생명공학부)
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