Rapid Detection of Escherichia coli in Fresh Foods Using a Combination of Enrichment and PCR Analysis
- Authors
- Choi, Yukyung; Lee, Sujung; Lee, Heeyoung; Lee, Soomin; Kim, Sejeong; Lee, Jeeyeon; Ha, Jimyeong; Oh, Hyemin; Lee, Yewon; Kim, Yujin; Yoon, Yohan
- Issue Date
- Aug-2018
- Publisher
- KOREAN SOC FOOD SCIENCE ANIMAL RESOURCES
- Keywords
- fresh meat; fresh-cut lettuce; Escherichia coli; enrichment; PCR
- Citation
- KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES, v.38, no.4, pp 829 - 834
- Pages
- 6
- Journal Title
- KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES
- Volume
- 38
- Number
- 4
- Start Page
- 829
- End Page
- 834
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/4405
- DOI
- 10.5851/kosfa.2018.e19
- ISSN
- 1225-8563
2636-0780
- Abstract
- The objective of this study was to determine the minimum enrichment time for different types of food matrix (pork, beef, and fresh-cut lettuce) in an effort to improve Escherichia coli detection efficiency. Fresh pork (20 g), beef (20 g), and fresh-cut lettuce (20 g) were inoculated at 1, 2, and 3 Log CFU/g of Escherichia coli. Samples were enriched in filter bags for 3 or 5 h at 44.5 degrees C, depending on sample type. E. coli cell counts in the samples were enriched in E. coli (EC) broth at 3 or 5 h. One milliliter of the enriched culture medium was used for DNA extraction, and PCR assays were performed using primers specific for uidA gene. To detect E. coli (uidA) in the samples, a 3-4 Log CFU/mL cell concentration was required. However, E. coli was detected at 1 Log CFU/g in fresh pork, beef, and fresh-cut lettuce after 5, 5, and 3-h enrichment, respectively. In conclusion, 5-h enrichment for fresh meats and 3-h enrichment for fresh-cut lettuce in EC broth at 44.5 degrees C, and PCR analysis using uidA gene-specific primers were appropriate to detect E. coli rapidly in food samples.
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