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Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns

Authors
Kim, Seong-MinLee, SeyeongKim, DongyoonKang, Dong-HeeYang, KisukCho, Seung-WooLee, Jin SeokChoi, Insung S.Kang, KyungtaeYoon, Myung-Han
Issue Date
May-2018
Publisher
TSINGHUA UNIV PRESS
Keywords
vertical silicon nanostructures; metal-assisted chemical etching; primary hippocampal neurons; neurite outgrowth; cytoskeletal dynamics
Citation
NANO RESEARCH, v.11, no.5, pp.2532 - 2543
Journal Title
NANO RESEARCH
Volume
11
Number
5
Start Page
2532
End Page
2543
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/4528
DOI
10.1007/s12274-017-1878-7
ISSN
1998-0124
Abstract
In this study, we report that height-controlled vertically etched silicon nano-column arrays (vSNAs) induce strong growth cone-to-substrate coupling and accelerate In vitroneurite development while preserving the essential features of initial neurite formation. Large-scale preparation of vSNAs with flat head morphology enabled the generation of well-controlled topographical stimulation without cellular impalement. A systematic analysis on topography-induced variations on cellular morphology and cytoskeletal dynamics was conducted. In addition, neurite development on the grid-patterned vSNAs exhibited preferential adhesion to the nanostructured region and outgrowth directionality. The arrangement of cytoskeletal proteins and the expression of a focal adhesion complex indicated that a strong coupling existed between the underlying nanocolumns and growth cones. Furthermore, the height-controlled nanocolumn substrates differentially modulated neurite polarization and elongation. Our findings provide an important insight into neuron-nanotopography interactions and their role in cell adhesion and neurite development.
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