Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns
- Kim, Seong-Min; Lee, Seyeong; Kim, Dongyoon; Kang, Dong-Hee; Yang, Kisuk; Cho, Seung-Woo; Lee, Jin Seok; Choi, Insung S.; Kang, Kyungtae; Yoon, Myung-Han
- Issue Date
- TSINGHUA UNIV PRESS
- vertical silicon nanostructures; metal-assisted chemical etching; primary hippocampal neurons; neurite outgrowth; cytoskeletal dynamics
- NANO RESEARCH, v.11, no.5, pp.2532 - 2543
- Journal Title
- NANO RESEARCH
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- In this study, we report that height-controlled vertically etched silicon nano-column arrays (vSNAs) induce strong growth cone-to-substrate coupling and accelerate In vitroneurite development while preserving the essential features of initial neurite formation. Large-scale preparation of vSNAs with flat head morphology enabled the generation of well-controlled topographical stimulation without cellular impalement. A systematic analysis on topography-induced variations on cellular morphology and cytoskeletal dynamics was conducted. In addition, neurite development on the grid-patterned vSNAs exhibited preferential adhesion to the nanostructured region and outgrowth directionality. The arrangement of cytoskeletal proteins and the expression of a focal adhesion complex indicated that a strong coupling existed between the underlying nanocolumns and growth cones. Furthermore, the height-controlled nanocolumn substrates differentially modulated neurite polarization and elongation. Our findings provide an important insight into neuron-nanotopography interactions and their role in cell adhesion and neurite development.
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