Modulation of Inducible Nitric Oxide Synthase Expression in LPS-Stimulated BV-2 Microglia by Prenylated Chalcones from Cullen corylifolium (L.) Medik. through Inhibition of I-kappa B alpha Degradationopen access
- Authors
- Kim, Do Hee; Li, Hua; Han, Yeong Eun; Jeong, Ji Hye; Lee, Hwa Jin; Ryu, Jae-Ha
- Issue Date
- Jan-2018
- Publisher
- MDPI
- Keywords
- Cullen corylifolium; Psoralea corylifolia; prenylated chalcone; nitric oxide; inducible nitric oxide synthase; prostaglandin E2; inhibitory-kappa B alpha
- Citation
- MOLECULES, v.23, no.1
- Journal Title
- MOLECULES
- Volume
- 23
- Number
- 1
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/4755
- DOI
- 10.3390/molecules23010109
- ISSN
- 1420-3049
- Abstract
- The overproduction of nitric oxide (NO) and prostaglandin E-2 (PGE(2)) by microglia may cause neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease. From the activity-guided purification of Cullen corylifolium (L.) Medik. (syn. Psoralea corylifolia L.), three prenylated chalcones were identified: isobavachalcone (1), bavachromene (2), and kanzonol B (3). These prenylated chalcones showed concentration-dependent inhibitory effects on NO and PGE(2) production in lipopolysaccharide (LPS)-activated microglia. Western blotting and RT-PCR analysis demonstrated that these prenylchalcones reduced the expression of protein and mRNA of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-activated microglia. Furthermore, three prenylated chalcones blocked the inhibitory-B (I-B) degradation and down-regulated nuclear factor B (NF-B) level of nucleus in LPS-stimulated BV-2 microglia. Therefore, these prenylated chalcones from Psoralea corylifolia may be beneficial for the treatment of neuro-inflammatory diseases by modulating iNOS and COX-2 expressions in activated microglial cells.
- Files in This Item
-
Go to Link
- Appears in
Collections - 약학대학 > 약학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.