Black ginseng activates Akt signaling, thereby enhancing myoblast differentiation and myotube growthopen access
- Authors
- Lee, Soo-Yeon; Go, Ga-Yeon; Vuong, Tuan Anh; Kim, Jee Won; Lee, Sullim; Jo, Ayoung; An, Jun Min; Kim, Su-Nam; Seo, Dong-Wan; Kim, Jin-Seok; Kim, Yong Kee; Kang, Jong-Sun; Lee, Sang-Jin; Bae, Gyu-Un
- Issue Date
- Jan-2018
- Publisher
- KOREAN SOC GINSENG
- Keywords
- Akt signaling; black ginseng; myoblast differentiation; myogenic conversion; myotube hypertrophy
- Citation
- JOURNAL OF GINSENG RESEARCH, v.42, no.1, pp 116 - 121
- Pages
- 6
- Journal Title
- JOURNAL OF GINSENG RESEARCH
- Volume
- 42
- Number
- 1
- Start Page
- 116
- End Page
- 121
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/4761
- DOI
- 10.1016/j.jgr.2017.08.009
- ISSN
- 1226-8453
2093-4947
- Abstract
- Background: Black ginseng (BG) has greatly enhanced pharmacological activities relative to white or red ginseng. However, the effect and molecular mechanism of BG on muscle growth has not yet been examined. In this study, we investigated whether BG could regulate myoblast differentiation and myotube hypertrophy. Methods: BG-treated C2C12 myoblasts were differentiated, followed by immunoblotting for myogenic regulators, immunostaining for a muscle marker, myosin heavy chain or immunoprecipitation analysis for myogenic transcription factors. Results: BG treatment of C2C12 cells resulted in the activation of Akt, thereby enhancing heterodimerization of MyoD and E proteins, which in turn promoted muscle-specific gene expression and myoblast differentiation. BG-treated myoblasts formed larger multinucleated myotubes with increased diameter and thickness, accompanied by enhanced Akt/mTOR/p70S6K activation. Furthermore, the BG treatment of human rhabdomyosarcoma cells restored myogenic differentiation. Conclusion: BG enhances myoblast differentiation and myotube hypertrophy by activating Akt/mTOR/p70S6k axis. Thus, our study demonstrates that BG has promising potential to treat or prevent muscle loss related to aging or other pathological conditions, such as diabetes. (C) 2017 The Korean Society of Ginseng, Published by Elsevier Korea LLC.
- Files in This Item
-
Go to Link
- Appears in
Collections - 약학대학 > 약학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.