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Enrichment Broth for the Detection of Campylobacter jejuni and Campylobacter coli in Fresh Produce and Poultry

Authors
Jo, YoumiOh, Hye-MinYoon, YohanLee, Sun-YoungHa, Ji-HyoungKim, Won-IlKim, Hwang-YongHan, SanghyunKim, Se-Ri
Issue Date
Nov-2017
Publisher
INT ASSOC FOOD PROTECTION
Keywords
Campylobacter coli; Campylobacter jejuni; Enrichment broth
Citation
JOURNAL OF FOOD PROTECTION, v.80, no.11, pp 1842 - 1850
Pages
9
Journal Title
JOURNAL OF FOOD PROTECTION
Volume
80
Number
11
Start Page
1842
End Page
1850
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/5059
DOI
10.4315/0362-028X.JFP-16-450
ISSN
0362-028X
1944-9097
Abstract
Although campylobacteriosis caused by Campylobacter jejuni and Campylobacter coli has been increasingly reported worldwide owing to the consumption of contaminated poultry and fresh produce, the current detection protocols are not selective enough to inhibit unspecific microbes other than these pathogens. Five antibiotics were separately added to Bolton broth, and the survival rates of 18 Campylobacter spp. and 79 non-Campylobacter spp. were evaluated. The survival rate of the non-Campylobacter spp. was the lowest in Bolton broth with rifampin (6.3%), followed by cefsulodin (12.7%), novobiocin (16.5%), and potassium tellurite and sulfamethozaxole (both 17.7%). Also the most effective concentration of rifampin was found to be 12.5 mg/L, which markedly inhibited non-Campylobacter strains while not affecting the survival of Campylobacter strains. After the Campylobacter spp. were enriched in Bolton broth supplemented with 12.5 mg/L rifampin (R-Bolton broth), CampyFood Agar (CFA) was found to be better in selectively isolating the pathogens in the enrichment broth than the International Organization for Standardization method of using modified charcoal cefoperazone deoxycholate agar (mCCDA) for this step. When applied to natural food samples-here, romaine lettuce, pepper, cherry tomato, Korean leek, and chicken-the R-Bolton broth-CFA combination decreased the number of false-positive results by 50.0, 4.2, 20.8, 50.0, and 94.4%, respectively, compared with the International Organization for Standardization method (Bolton broth-mCCDA combination). These results demonstrate that the combination of R-Bolton broth and CFA is more efficient in detecting C. jejuni and C. coli in poultry and fresh produce and thus should replace the Bolton broth-mCCDA combination.
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