Eupatilin with PPAR alpha agonistic effects inhibits TNF alpha-induced MMP signaling in HaCaT cells
- Authors
- Jung, Yujung; Kim, Jin-Chul; Choi, Yongsoo; Lee, Sullim; Kang, Ki Sung; Kim, Yong Kee; Kim, Su-Nam
- Issue Date
- Nov-2017
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Eupatilin; PPAR alpha; MMPs; NF-kappa B; AP-1
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.493, no.1, pp 220 - 226
- Pages
- 7
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 493
- Number
- 1
- Start Page
- 220
- End Page
- 226
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/5068
- DOI
- 10.1016/j.bbrc.2017.09.043
- ISSN
- 0006-291X
1090-2104
- Abstract
- Eupatilin (5,7-dihydroxy-3,4,6-trimethoxyflavone) is a flavonoid compound exhibiting several beneficial biological activities, including neuroprotection, anti-cancer, antinociception, chondroprotection, anti oxidation, and anti-inflammation. Our previous study demonstrated that eupatilin specifically activates peroxisome proliferator-activated receptor alpha (PPAR alpha) through direct binding. The PPAR subfamily includes ligand-dependent transcription factors that consist of three isotypes: PPAR alpha, PPAR beta/delta, and PPAR gamma. All isotypes are involved in inflammation, epidermal proliferation/differentiation and skin barrier function. Among them, PPAR alpha regulates lipid and glucose metabolism and skin homeostasis. In this study, we confirm that the ability of eupatilin as a PPAR alpha activator significantly inhibited tumor necrosis factor-alpha (TNF alpha)-induced matrix metalloproteinase (MMP)-2/-9 expression and proteolytic activity in HaCaT human epidermal keratinocytes. Furthermore, we found that eupatilin subsequently suppressed I kappa B alpha phosphorylation, blocked NF-kappa B p65 nuclear translocation and down-regulated MAPK/AP-1 signaling via PPAR alpha activation. Taken together, our data suggest that eupatilin inhibits TNF alpha-induced MMP-2/-9 expression by suppressing NF-kappa B and MAPK/AP-1 pathways via PPAR alpha. Our findings suggest the usefulness of eupatilin for preventing skin aging. (C) 2017 Elsevier Inc. All rights reserved.
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