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Suppressive effects of hesperidin on Th2-associated cytokines expression in RBL-2H3 cells

Authors
정화현김순례표명윤
Issue Date
Jun-2013
Publisher
한국생약학회
Keywords
Antiallergy; Hesperidin; IL-13; IL-4; RBL-2H3
Citation
Korean Journal of Pharmacognosy, v.44, no.2, pp 104 - 109
Pages
6
Journal Title
Korean Journal of Pharmacognosy
Volume
44
Number
2
Start Page
104
End Page
109
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/6377
ISSN
0253-3073
Abstract
Hesperidin (HES), a flavonone glycoside isolated from the citrus fruits such as lemons and oranges, has been reported to have many biological properties including antiinflammatory, antioxidant, and antiallergy activities. In this study, we focused on the action of HES modulating Th2-associated cytokines such as IL-4 and IL-13 expression in PMA/ionomycin (PI)- stimulated rat basophilic leukemia (RBL-2H3) cells. The production of IL-4 and IL-13 was quantified by ELISA and the mRNA expression was detected by using RT-PCR assay. In addition, western blot analysis was performed to determine the transcription factors involved in the cytokine expression. We found that HES significantly decreased PI-induced IL-4 and IL-13 productions and also decreased the level of mRNA in a dose-dependent manner. Furthermore, western blot analysis of the transcription factors implied that HES down-regulated the protein level of c-Jun and c-Fos, which are the activating protein 1 (AP- 1) family and nuclear factor-kappaB (NF-κB) characterized as a transcription factors related to the Th2-associated cytokine expression. Taken together, our data showed that the action of HES responsible for antiallergy activities is based on suppression of Th2-associated cytokines through inhibition of AP-1 and NF-κB transcription factors.
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