더덕 물 추출물의 경구 투여가 마우스 면역 세포 활성에 미치는 효과Effects of Plant water Extract Codonopsis Lanceolatae on Mouse Immune Cell Activation Ex Vivo
- Other Titles
- Effects of Plant water Extract Codonopsis Lanceolatae on Mouse Immune Cell Activation Ex Vivo
- Authors
- 류혜숙; 김경옥; 김현숙
- Issue Date
- Apr-2009
- Publisher
- 한국영양학회
- Keywords
- splenocyte proliferation; IL-1β; IL-6; TNF-α; cytokine
- Citation
- Journal of Nutrition and Health, v.42, no.3, pp 207 - 212
- Pages
- 6
- Journal Title
- Journal of Nutrition and Health
- Volume
- 42
- Number
- 3
- Start Page
- 207
- End Page
- 212
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/7646
- ISSN
- 2288-3886
2288-3959
- Abstract
- Codonopsis lanceolatae has been used as one of the traditional remedies as well as food source. However, few
studies on their immunomodulating effects have been reported. We previously reported that ex vivo supplementation of
Codonopsis lanceolatae water extracts enhanced splenocyte proliferation compared to the control group. In order to
elucidate its ex vivo effect, six to seven week old balb/c mice were fed ad libitum on a chow diet and water extracts of
Codonopsis lanceolatae were orally administrated every other day for four weeks at two different concentrations (50
and 500 mg/kg B.W.). After preparing the single cell suspension, the proliferation of splenocytes was determined by
MTT (3-[4,5-dimethylthiazol-2-y]-2,5-diphenyl terazolium bromide) assay. The production of cytokine (IL-1β, IL-6,
TNF-α), secreted by macrophages stimulated with LPS or not, was detected by ELISA assay using a cytokine kit.
After 48 hrs of incubation with the mitogen (ConA or LPS) stimulation, the mice splenocyte proliferation in experimental
group was statistically increased at two different concentrations than that in control group. The cytokines production
was more significantly enhanced at the lower supplementation (500 mg/kg B.W.) group rather than higher concentration
(500 mg/kg B.W.) compared to the control group. The results of this study may suggest that the supplementation
of water extract of plant mixture could regulate the immune function by increasing the splenocyte proliferation
and enhance the immune function through regulating cytokine production capacity by activated macrophages in mice.
(Korean J Nutr 2009; 42(3): 207 ~ 212)
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