Detailed Information

Cited 0 time in webofscience Cited 14 time in scopus
Metadata Downloads

Paeonia japonica, Houttuynia cordata, and Aster scaber Water Extracts Induce Nitric Oxide and Cytokine Production by Lipopolysaccharide-Activated Macrophages

Authors
Kim, JinPark, Chang-ShinLim, YunsookKim, Hyun-Sook
Issue Date
Apr-2009
Publisher
MARY ANN LIEBERT, INC
Keywords
Aster scaber; cyclooxygenase-2; cytokine; Houttuynia cordata; nitric oxide; Paeonia japonica
Citation
JOURNAL OF MEDICINAL FOOD, v.12, no.2, pp.365 - 373
Journal Title
JOURNAL OF MEDICINAL FOOD
Volume
12
Number
2
Start Page
365
End Page
373
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/7650
DOI
10.1089/jmf.2008.1013
ISSN
1096-620X
Abstract
Natural products are increasingly recognized as potential targets for drug discovery and development. We previously reported that Paeonia japonica, Houttuynia cordata, and Aster scaber enhanced macrophage activation both in vitro and in vivo. In the present study we investigated the immunomodulating effects of these plants on lipopolysacharide (LPS)-stimulated macrophages. An aqueous extract of each plant was administered to female BALB/c mice every other day for 4 weeks. Peritoneal macrophages were then collected and incubated to examine the immunoreactivity of macrophages against LPS at different time points. The expression levels of inducible nitric oxide (NO) synthetase (iNOS), cyclooxygenase (COX)-2, and inhibitory factor kappa B alpha (I kappa B alpha) proteins and the production of NO metabolite (nitrite), prostaglandin (PG) E-2, and the pro-inflammatory cytokines interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha were determined in the activated macrophages treated with extracts from each plant individually or combined. High levels of pro-inflammatory cytokines were produced by A. scaber-, P. japonica-, and H. cordata-treated macrophages following 24 hours of LPS stimulation. P. japonica, H. cordata, and A. scaber treatment also induced the production of nitrate by LPS-treated macrophages. Induction of iNOS mRNA and protein was also different in each group. PGE(2) secretion was up-regulated by all extract-treated macrophages at early time points; however, no significant differences were observed between the groups by 8 hours post-LPS stimulation. Treatment with A. scaber extract resulted in the highest levels of I kappa B alpha degradation. Our findings illustrate that the natural plant products P. japonica, H. cordata, and A. scaber may enhance immune function by modulating ex vivo pro-inflammatory cytokine and NO production as well as the expression of iNOS and COX-2.
Files in This Item
Go to Link
Appears in
Collections
생활과학대학 > 식품영양학과 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Hyun Sook photo

Kim, Hyun Sook
생활과학대학 (식품영양학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE