Detailed Information

Cited 0 time in webofscience Cited 51 time in scopus
Metadata Downloads

Ascorbate (vitamin C) induces cell death through the apoptosis-inducing factor in human breast cancer cells

Authors
Hong, Seung-WooJin, Dong-HoonHahm, Eun-SilYim, Sei-HyunLim, Jong-SeokKim, Keun-IlYang, YoungLee, Soo-SukKang, Jae-SeungLee, Wang-JaeLee, Won-KeunLee, Myeong-Sok
Issue Date
Oct-2007
Publisher
SPANDIDOS PUBL LTD
Keywords
ascorbate (vitamin C); apoptosis-inducing factor; caspase; cell death; Ca2+
Citation
ONCOLOGY REPORTS, v.18, no.4, pp.811 - 815
Journal Title
ONCOLOGY REPORTS
Volume
18
Number
4
Start Page
811
End Page
815
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/8342
DOI
10.3892/or.18.4.811
ISSN
1021-335X
Abstract
Although ascorbate (Vitamin C) has been shown to inhibit cell growth and induce cell death in variety of cancer cells, results reported in other studies are inconsistent with this conclusion. It was previously reported that ascorbate induces apoptosis in human breast cancer cells. However, the molecular mechanism for this is not clear. In this study, we demonstrate that ascorbate induces cell death through the apoptosis-inducing factor (AIF) in the human breast cancer cell lines, SK-BR3 and Hs578T, but not in a normal breast cell line, Hs578. Ascorbate treatment caused the nuclear translocation of AIF, which is retained in the mitochondria in healthy cells, but caspase cleavage is not induced. Moreover, MG132, an inhibitor of AIF release from mitochondria, blocked the induction of cell death. Furthermore, cells that had been treated with human AIF-specific siRNA resisted cell death induced by ascorbate, implying that the translocation of AIF from mitochondria to the nucleus is responsible for ascorbate-mediated cell death. Therefore, these results suggest that ascorbate activates a caspase-independent and AIF-mediated cell death pathway in human breast cancer cells, SK-BR3, and Hs578T.
Files in This Item
Go to Link
Appears in
Collections
이과대학 > 생명시스템학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Lee, Myeong Sok photo

Lee, Myeong Sok
이과대학 (생명시스템학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE