Selective Enrichment of Conjugated Linoleic Acid Isomers in Their Mixtures Using Combined Chemical and Enzymatic Methods
- Authors
- Kim, Jiwon; Chung, Min-Yu; Choi, Hee-Don; Choi, In-Wook; Kim, In-Hwan; Chun, Hyang Sook; Kim, Byung Hee
- Issue Date
- Apr-2017
- Publisher
- WILEY
- Keywords
- Urea crystallization; Esterification; Candida rugosa lipase; Recirculating packed-bed reactor; trans-10,cis-12 Conjugated linoleic acid; cis-9,trans-11 Conjugated linoleic acid
- Citation
- JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, v.94, no.4, pp 577 - 585
- Pages
- 9
- Journal Title
- JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
- Volume
- 94
- Number
- 4
- Start Page
- 577
- End Page
- 585
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/8578
- DOI
- 10.1007/s11746-017-2970-6
- ISSN
- 0003-021X
1558-9331
- Abstract
- The aim of this study was to selectively enrich t10,c12-conjugated linoleic acid (t10,c12-CLA) and c9,t11-CLA in commercial CLA mixtures using a combination of urea crystallization and lipase-catalyzed esterification. The objective of the urea fractionation is to remove saturated and monounsaturated fatty acids (FA) from the CLA mixtures. CLA-enriched free FA (FFA) mixtures containing 53.8 wt% t10,c12-CLA and 39.1 wt% c9,t11-CLA were produced from the CLA mixtures containing similar to 34 wt% each of the two CLA isomers by a urea crystallization using methanol and the urea-to-FA weight ratio of 2.5:1. The CLA-enriched FFA mixtures were partially esterified with dodecan-1-ol in a recirculating packed-bed reactor using an immobilized lipase from Candida rugosa to further enrich the t10,c12-CLA and c9,t11-CLA in an FFA fraction and an FA dodecyl ester fraction, respectively, under the optimal conditions, i.e., temperature, 20 A degrees C; FA-to-dodecan-1-ol molar ratio, 1:1; water content, 2 wt% of total substrates; residence time, 5 min; and reaction time, 24 h (for t10,c12-CLA enrichment) and 12 h (for c9,t11-CLA enrichment). After the reaction, an FFA fraction with 72.6 wt% t10,c12-CLA was obtained. Another FFA fraction with 62.0 wt% c9,t11-CLA was recovered after the saponification of the FA dodecyl ester fraction. The yields of t10,c12-CLA and c9,t11-CLA in the FFA fractions were 43.6 and 21.5 wt%, respectively, based on their initial weights in the CLA mixtures.
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