3',4',7,8-Tetrahydroxyflavone inhibits RANKL-induced osteoclast formation and bone resorption
- Authors
- Kang, J. H.; Jung, H.; Sy, Ji; Yim, M.
- Issue Date
- Mar-2017
- Publisher
- GOVI-VERLAG PHARMAZEUTISCHER VERLAG GMBH
- Citation
- PHARMAZIE, v.72, no.3, pp 161 - 166
- Pages
- 6
- Journal Title
- PHARMAZIE
- Volume
- 72
- Number
- 3
- Start Page
- 161
- End Page
- 166
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/8628
- DOI
- 10.1691/ph.2017.6845
- ISSN
- 0031-7144
- Abstract
- Osteoclasts, which are specialized bone multinuclear cells, are responsible for bone lytic diseases such as osteoporosis. 3',4',7,8-tetrahydroxyflavone is a flavonoid from Acacia confusa. In the present study, we found that 3',4',7,8-tetrahydroxyflavone markedly inhibited receptor activator of nuclear factor kappa B ligand (RANKL)induced osteoclastic differentiation from mouse bone marrow-derived macrophages (BMMs). 3',4',7',8-tetrahydroxyflavone also reduced the mRNA expression levels of osteoclastic marker genes including the calcitonin receptor (CTR) and cathepsin K. In addition, 3',4',7',8-tetrahydroxyflavone decreased the bone resorption activity of osteoclasts on dentin slices. We found that 3',4',7',8-tetrahydroxyflavone inhibited RANKL-induced expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1), a key transcription factor of osteoclast differentiation. Furthermore, ectopic overexpression of a constitutively active form of NFATc1 completely rescued the anti-osteoclastogenic effect of 3',4',7',8-tetrahydroxyflavone, suggesting that the anti-osteoclastogenic effect was mainly attributed to the reduction in NFATc1 expression. Taken together, our data suggest that 3',4',7',8-tetrahydroxyflavone inhibits osteoclast differentiation and bone loss and may therefore be considered a promising drug candidate for treating or preventing bone-lytic diseases.
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