Suppressor of bri1-120 mutant allele revealed interrelated and independent actions of brassinosteroid and light signaling
- Authors
- Shang, Yun; Fu, Minjie; Nam, Kyoung Hee
- Issue Date
- Dec-2016
- Publisher
- SPRINGER HEIDELBERG
- Keywords
- Brassinosteroid; BRI1; Light; PHYTOCHROME B; Suppressor screening
- Citation
- JOURNAL OF PLANT BIOLOGY, v.59, no.6, pp 594 - 602
- Pages
- 9
- Journal Title
- JOURNAL OF PLANT BIOLOGY
- Volume
- 59
- Number
- 6
- Start Page
- 594
- End Page
- 602
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/9324
- DOI
- 10.1007/s12374-016-0366-z
- ISSN
- 1226-9239
1867-0725
- Abstract
- Brassinosteroids (BRs) are plant hormones that affect diverse aspects of plant development. Various BR-biosynthetic or BR-signaling mutants contribute to BR functions and signaling events in many plant species. The BR receptor brassinosteroid-Insensitive 1 (BRI1) plays critical roles in BR signaling. We previously identified a weak bri1 mutant allele, bri1-120, that has a mutation site in the extracellular domain of BRI1. Here, genetic suppressor screening revealed that a PHYB gene mutation led to suppression of ethyl methanesulfonate (EMS)-mutagenized bri1-120. The morphology of bri1-120phyB-1 indicated that compact and rounded phenotypes of bri1-120 were suppressed. However, BR sensitivity of the bri1-120phyB-1 was only recovered in hypocotyl elongation, and overexpression of PHYB in bri1-120 did not enhance bri1-120 phenotypes. To further investigate the relationship between BR and light signalings, we examined the seed germination pattern and hypocotyl growth of bri1-120phyB-1 as compared to that of each single mutant under various light conditions. Seed germination in bri1-120phyB-1 was higher than in both the single mutants. Hypocotyl length in bri1-120phyB-1 was intermediate between that of bri1-120 and phyB-1, whereas sensitivity to red light in bri1-120phyB-1 remained the same as in phyB-1. These results suggest that BR and light signalings affect diverse cellular responses both together and independently, depending on the specific cellular processes.
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