Neuroprotective effect of mesenchymal stem cell through complement component 3 downregulation after transient focal cerebral ischemia in mice
- Authors
- Jung, Hye-Seon; Jeong, Si-Yeon; Yang, Jiwon; Kim, So-Dam; Zhang, Baojin; Yoo, Hyun Seung; Song, Sun U.; Jeon, Myung-Shin; Song, Yun Seon
- Issue Date
- Oct-2016
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- Mesenchymal stem cell; Complement component 3; Neuroprotection; Cerebral ischemia; Oxygen glucose deprivation
- Citation
- NEUROSCIENCE LETTERS, v.633, pp 227 - 234
- Pages
- 8
- Journal Title
- NEUROSCIENCE LETTERS
- Volume
- 633
- Start Page
- 227
- End Page
- 234
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/9400
- DOI
- 10.1016/j.neulet.2016.09.032
- ISSN
- 0304-3940
1872-7972
- Abstract
- Bone marrow-derived mesenchymal stem cells (MSCs) are used in stroke treatment despite the poor understanding of its mode of action. The immune suppressive and anti-inflammatory properties of MSCs possibly play important roles in regulating neuroinflammation after stroke. We investigated whether MSCs reduce the inflammatory complement component 3 (C3) levels, thus, providing neuroprotection during stroke. Mice were subjected to transient focal cerebral ischemia (tFCI), after which MSCs were intravenously injected. The infarct volume of the brain was reduced in MSC-injected tFCI mice, and C3 expression was significantly reduced in both the brain and the blood. Additionally, the profiles of other inflammatory mediators demonstrated neuroprotective changes in the MSCs-treated group. In order to analyze the effect of MSCs on neurons during cerebral ischemia, primary cortical neurons were co cultured with MSCs under oxygen-glucose deprivation (OGD). Primary neurons co -cultured with MSCs exhibited reduced levels of C3 expression and increased protection against OGD, indicating that treatment with MSCs reduces excessive C3 expression and rescues ischemia-induced neuronal damage. Our finding suggests that reduction of C3 expression by MSCs can help to ameliorate ischemic brain damage, offering a new neuroprotective strategy in stroke therapy. (C) 2016 Published by Elsevier Ireland Ltd.
- Files in This Item
-
Go to Link
- Appears in
Collections - 약학대학 > 약학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.