IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells
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초록

Aurora B activation is triggered at the mitotic entry and required for proper microtubule-kinetochore attachment at mitotic phase. Therefore, Aurora B should be in inactive form in interphase to prevent aberrant cell cycle progression. However, it is unclear how the inactivation of Aurora B is sustained during interphase. In this study, we find that IK depletion-induced mitotic arrest leads to G2 arrest by Aurora B inhibition, indicating that IK depletion enhances Aurora B activation before mitotic entry. IK binds to Aurora B, and colocalizes on the nuclear foci during interphase. Our data further show that IK inhibits Aurora B activation through recruiting PP2A into IK and Aurora B complex. It is thus believed that IK, as a scaffold protein, guides PP2A into Aurora B to suppress its activity in interphase until mitotic entry.

키워드

Aurora BCell cycleIKInterphaseMitosisPP2AProtein REDSPINDLE ASSEMBLY CHECKPOINTKINASE-ACTIVITYPROTEIN PHOSPHATASE-1CENTROSOME MATURATIONCYTOKINE IKA KINASEC KINASECLASS-IIMITOSISACTIVATION
제목
IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells
저자
Lee, SunyiJeong, Ae LeePark, Jeong SuHan, SoraJang, Chang-YoungKim, Keun IlKim, YonghwanPark, Jong HoonLim, Jong-SeokLee, Myung SokYang, Young
DOI
10.1007/s00018-016-2162-9
발행일
2016-09
유형
Article
저널명
Cellular and Molecular Life Sciences
73
17
페이지
3375 ~ 3386